How to understand the Homogenizer classification and feature?

Update:23 Apr 2018

How to understand the Homogenizer classification and feature?

There are three main types of homogenizers commonly used in laboratories: ultrasonic homogenizers, probe-sharp homogenizers, and stroking homogenizers. Each type of homogenizer has its scope of application, advantages, and disadvantages. Ultrasonic Dispersion The editor introduced three types of homogenizers to each one.

1.Ultrasonic homogenizer

Principle: Using sound waves and ultrasonic waves, the principle of rapid alternating compression and expansion when working with objects. When the material is under the action of ultrasonic waves, the material and liquid will be expanded by the bubble when it is in the expansion half cycle; when it is in the compression half cycle, the bubble will shrink when the pressure changes greatly and the pressure is lower than the pressure At that time, the compressed air bubbles collapse sharply, and a "hole" phenomenon occurs in the feed liquid. This phenomenon disappears with the change of pressure and the unbalance of the external pressure. At the moment when the "hole" disappears, the liquid The surroundings cause very large pressure and temperature increase, and play a very complicated and powerful mechanical stirring effect to achieve the purpose of homogenization.

Scope of application: mashing and cell lysis of various tissues, extraction of organelles, nucleic acids and proteins, and emulsification and homogenization of other industrial samples.

Advantages: easy to use, by changing the different probes, can handle different amounts of sample; emulsification, homogenization effect is good, suitable for single sample operation.

Disadvantages: Can not process multiple samples at the same time, different samples need to be replaced or cleaned probes, increase the chance of cross-contamination between samples; have certain influence on biological samples with special requirements.

2.Probe rotary blade homogenizer

Principle: This type is used for the purpose of separating, mixing, crushing and homogenizing by grinding and rotating in a homogenizer. It is suitable for processing tough samples.

Scope of application: It is used to disperse animal/plant tissue, with the lysate for extraction of nucleic acids, proteins, etc. It can also be used to make suspensions/emulsions and the like in industrial resins and pigments.

Advantages: low speed, large torque, no noise and so on. Easy to use, by changing the different probes, Ultrasonic Cutting Machine can handle different amounts of sample, easy to operate, more suitable for single sample operation.

Disadvantages: Can not process multiple samples at the same time, different samples need to be replaced or cleaned probes, increase the chance of cross-contamination between samples; For the treatment of thick-walled samples such as bacteria, yeast and other fungi do not consider this kind of homogenizer.

3.Slamming homogenizer

Principle: Slamming on the bag through the slamming board. The resulting pressure can crush and mix the contents of the bag. The bead homogenizer grinds the sample and the corresponding beads in a test tube by three-dimensional high-speed rotation and vibration and grinds the sample by high-speed tapping of the beads, and homogeneously breaks the sample.

Scope of application: Wide range of applications, can be used for the fragmentation of animal and plant tissues, algae, bacteria, yeast, fungi or molds and various spores, DNA/RNA, protein extraction.

Advantages: Efficient treatment of stubborn samples including bones, spores, soil, etc. Each homogenizer in the homogenizer is equipped with a homogenizer knife to avoid cross-contamination. It is easy and efficient to handle, and it is better to handle brittle samples. .

Disadvantages: can not handle large volume samples, a single sample of a single processing volume is generally less than 1.5ml, and need to use with the corresponding homogenized bags, so supplies and equipment investment higher.